Hybrids may also be produced by a technique called protoplast fusion.
2.
Protoplasts may also be used for plant breeding, using a technique called protoplast fusion.
3.
This release is then followed by protoplast fusion ( conjugation ) leading to formation of a diploid zygospore.
4.
Code-named Y-1, the plant is the product of state-of-the-art breeding techniques, including processes known as protoplast fusion and hybrid sorting.
5.
The classical plant breeder may also make use of a number of " in vitro " techniques such as protoplast fusion, embryo rescue or mutagenesis ( see below ) to generate diversity and produce hybrid plants that would not exist in nature.
6.
These specific genetic engineering techniques include various recombinant DNA and RNA methods such as micro-injection, electroporation, micro-encapsulation, liposome fusion, protoplast fusion or other " hybridization techniques that overcome natural physiological, reproductive or recombination barriers, where the donor cells or protoplasts do not fall within the same taxonomic family, in a way that does not occur by natural multiplication or natural recombination ."
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